Mass Spectrometry
The Mass Spectrometry Research Facility at DiSC consists of ten main instruments.
They include the coupled setup with Gas Cromatography (GC/MS), High Pressure Liquid Cromatography (HPLC/MS), Matrix-Assisted Laser Desorption/Ionisation-Time of Flight (MALDI-TOF) and Inductively Coupled Plasma (ICP) also coupled with an ionic chromatography (IC) system. Interfaced ionisation sources for LC instruments include Electro Spray Ionisation (ESI), Atmospheric Pressure Chemical Ionisation (APCI) and Atmospheric Pressure Photo Ionisation (APPI).
A wide range of molecular systems can be investigated, including elements and metals speciation, organic molecules, peptides, proteins, bio-polymers, synthetic polymers.
The facility also provides commercial services to industry where possible.
Some of the mass spectrometers housed at DiSC can be cited here:
Thermo Scientific™ Q Exactive™ Hybrid Quadrupole-Orbitrap Mass Spectrometer
The Thermo Scientific™ Q Exactive™ Hybrid Quadrupole-Orbitrap Mass Spectrometer identiftes, quantifies and confirms more compounds rapidly and with confidence. This LC-MS/MS system combines quadruple precursor ion selection with high-resolution, accurate-mass (HRAM) Orbitrap detection to deliver exceptional performance and versatility. The Q Exactive Mass Spectrometer is equally useful for untargeted or targeted screening and a broad range of qualitative and quantitative applications in drug discovery, proteomics, environmental and food safety, clinical research and forensic toxicology.
contacts: paolo.pastore@unipd.it
location at DiSC: 210-05-?
Agilent 6520 Accurate-Mass Q-TOF LC/MS system
The Agilent 6520 Accurate-Mass Q-TOF LC/MS system provide superior data quality and advanced analytical capabilities for profiling, identifying, characterizing, and quantifying low molecular-weight compounds and biomolecules with confidence.
contacts: paolo.pastore@unipd.it
location at DiSC: 210-05-?
AB SCIEX MALDI-TOF 4800 Plus
This combination of Matrix-Assisted Laser Desorption/Ionization source with a Time-Of-Flight mass analyzer offers high performance and flexibility for the multi-user core laboartory, allowing for gel spot analysis, protein biomarker discovery and MALDI mass spectrometry imaging, fast peptide and protein identification, high-sensitivity of MS and MS/MS data on small molecules as well as peptides and proteins, in any type of tissue.
contacts: cristiano.zonta@unipd.it
location at DiSC: 170-00-016
UPLC-DAD-MS Agilent Technologies 1290 Infinity
The equipment includes a high performance chromatographic separation unit (HPLC) interfaced to an Agilent 1290 Infinity Diode Array Detector (DAD) and a 6130 single quadrupole ESI/MS. The equipment further includes an autosampler, binary pumping system and a temperature control unit.
contacts: leonard.prins@unipd.it
location at DiSC: 170-00-016
Agilent Technologies MSD SL Trap and HPLC 1100 series
contacts: ester.marotta@unipd.it
location at DiSC: 170-00-016
