Mass Spectrometry

The Mass Spectrometry Research Facility at DiSC consists of seven main instruments.
They include the coupled setup with Gas Cromatography (GC/MS), High Pressure Liquid Cromatography (HPLC/MS) and Matrix-Assisted Laser Desorption/Ionisation-Time of Flight (MALDI_TOF). Interfaced ionization techniques and inlet systems include Electro Spray Ionisation (ESI), Atmospheric Pressure Chemical Ionisation (APCI) and Atmospheric Pressure Photo Ionisation (APPI).
A wide range of molecular systems can be investigated, including organic molecules, peptides, proteins, bio-polymers, synthetic polymers.
The facility also provides commercial services to industry where possible.

Some of the mass spectrometers housed at DiSC can be cited here:

  Thermo Scientific™ Q Exactive™ Hybrid Quadrupole-Orbitrap Mass Spectrometer

The Thermo Scientific™ Q Exactive™ Hybrid Quadrupole-Orbitrap Mass Spectrometer identiftes, quantifies and confirms more compounds rapidly and with confidence. This LC-MS/MS system combines quadruple precursor ion selection with high-resolution, accurate-mass (HRAM) Orbitrap detection to deliver exceptional performance and versatility. The Q Exactive Mass Spectrometer is equally useful for untargeted or targeted screening and a broad range of qualitative and quantitative applications in drug discovery, proteomics, environmental and food safety, clinical research and forensic toxicology.

contacts: paolo.pastore@unipd.it
location at DiSC: 210-05-?

  Agilent 6520 Accurate-Mass Q-TOF LC/MS system

The Agilent 6520 Accurate-Mass Q-TOF LC/MS system provide superior data quality and advanced analytical capabilities for profiling, identifying, characterizing, and quantifying low molecular-weight compounds and biomolecules with confidence.

contacts: paolo.pastore@unipd.it
location at DiSC: 210-05-?

  AB SCIEX MALDI-TOF 4800 Plus

This combination of Matrix-Assisted Laser Desorption/Ionization source with a Time-Of-Flight mass analyzer offers high performance and flexibility for the multi-user core laboartory, allowing for gel spot analysis, protein biomarker discovery and MALDI mass spectrometry imaging, fast peptide and protein identification, high-sensitivity of MS and MS/MS data on small molecules as well as peptides and proteins, in any type of tissue.

contacts: cristiano.zonta@unipd.it
location at DiSC: 170-00-016

  Thermo Finnigan LCQ Deca XP Max LC/MS

 

contacts: ester.marotta@unipd.it
location at DiSC: 170-00-016

  UPLC-DAD-MS Agilent Technologies 1290 Infinity

The equipment includes a high performance chromatographic separation unit (HPLC) interfaced to an Agilent 1290 Infinity Diode Array Detector (DAD) and a 6130 single quadrupole ESI/MS. The equipment further includes an autosampler, binary pumping system and a temperature control unit.

contacts: leonard.prins@unipd.it
location at DiSC: 170-00-016

  Agilent Technologies MSD SL Trap and HPLC 1100 series

 

contacts: ester.marotta@unipd.it
location at DiSC: 170-00-016

  Fison TRIO 1000

 

 

contacts: ester.marotta@unipd.it
location at DiSC: 170-00-016